The RING-H2 gene <i>LdXERICO</i> plays a negative role in dormancy release regulated by low temperature in <i>Lilium davidii</i> var. <i>unicolor</i>

Abstract Dormancy regulation is the basis of sustainable development lily industry. Therefore, basic research on dormancy crucial for innovation cultivation and breeding. Previous studies revealed that release largely depends abscisic acid (ABA) degradation. However, key genes potential regulatory network remain unclear. We used exogenous ABA inhibitors to elucidate effect dormancy. Based results weighted gene coexpression analysis (WGCNA), hub LdXERICO was identified in modules highly related endogenous ABA, a large number coexpressed were identified. induced by expressed at higher levels tissues with vigorous physiological activity. Silencing increased low-temperature sensitivity bulblets accelerated bulblet sprouting. rescued insensitivity xerico mutants during seed germination Arabidopsis, suggesting it promotes supporting overexpression bulblets. The significant increase transgenic Arabidopsis expressing indicated played role promoting synthesis. generated three lines overexpressing LdICE1 thaliana showed contrast LdXERICO, positively regulated release. Finally, qRT–PCR confirmed epistatic propose an essential through ABA-related pathway, has complex involving temperature signals. This study provides theoretical further exploring mechanism bulb